Composite

Part:BBa_K4583053:Design

Designed by: Suiru Lu   Group: iGEM23_SDU-CHINA   (2023-10-05)


PHB production Regulation: PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2740
    Illegal BglII site found at 3565
    Illegal BamHI site found at 1791
    Illegal XhoI site found at 1511
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2046
    Illegal NgoMIV site found at 2117
    Illegal NgoMIV site found at 2717
    Illegal NgoMIV site found at 3029
    Illegal NgoMIV site found at 3308
    Illegal NgoMIV site found at 3960
    Illegal NgoMIV site found at 3982
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5826
    Illegal SapI.rc site found at 769


Design Notes

PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz



Source

PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz


References